Cobas Taqman Hiv 1 Test V2 0 Manual

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Free download cobas taqman hiv 1 test v2 0 manual. The COBAS ® TaqMan ® HIV-1 Test, version (v) For Use With The High Pure System is an in vitro nucleic acid amplification test for the quantitation of Human Immunodeficiency Virus Type 1 (HIV-1) RNA in human plasma, using the High Pure System Viral Nucleic Acid Kit for manual specimen preparation and the COBAS ® TaqMan ® 48 Analyzer for automated amplification and detection.

Testsysteme: COBAST AmpliPrep: automatisierte Probenvorbereitung COBAS T TaqMan 48 oder COBAS TaqManT: Real-Time-PCR Testzeit: ca. 6 h, inklusive Probenvorbereitung Bestellinformationen COBAS T AmpliPrep / COBAS TaqMan HIV-1 Test, v Bestellnummer: 05 COBAST AmpliPrep / COBAS TaqManT 48 COBAST AmpliPrep / COBAS TaqManT System.

COBAS TaqMan 48 HIV-1 Test, v Bestimmung der Viruslast mit dem High Pure System zur manuellen Probenvorbereitung Dual-Target-Detection GAG-Target: 4 Primer amplifizieren ein bp-Produkt 5’-NCR-Target: 3 Primer amplifizieren ein bp-Produkt Target-spezifische Sonden: beide Sonden sind FAM-markiert und er- geben summiert ein Fluoreszenzsignal Testeigenschaften.

The COBAS ® AmpliPrep/COBAS ® TaqMan ® HIV-1 Test, version (v) is an in vitro nucleic acid amplification test for the quantitation of human immunodeficiency virus type 1 (HIV-1) RNA in human plasma using the COBAS ® AmpliPrep Instrument for automated specimen processing and the COBAS ® TaqMan ® Analyzer or COBAS ® TaqMan ® 48 Analyzer for automated amplification and.

Cobas Ampliprep/Cobas TaqMan HIV-1 v Assay: Consequences at the Cohort Level. Analytical characteristics and comparative evaluation of Aptima HIV-1 Quant Dx assay with Ampliprep/COBAS TaqMan HIV-1 test v Angelos. Hatzakis1,2,4*, Helen Papachristou1, Sangeetha J. Nair3, Jacqueline Fortunko3, Tracy Foote3, HeeCheol Kim3, Tashi L. Peling3 and Andrew J. Worlock3 Abstract Background: Quantitation of HIV-RNA is critically important for diagnosis, prognosis.

The COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 Test is an in vitro nucleic acid amplification test for the quantitation of Human Immunodeficiency Virus Type 1 (HIV-1) RNA in human plasma using the. Running the COBAS Ampliprep/COBAS TaqMan HIV-1 Qualitative Test 1. Go to Tasks – Assays on the menu bar or click the Assays () button on the LDMS toolbar. 2. Click the plus sign (+) next to the DNA PCR category. 3. Click COBAS TaqMan HIV-1 Qual. (See Figure 1.). Figure 1: Assays. 4. Click Select Assay. (See Figure 2.) Figure 2: Assay File Size: KB.

The COBAS ® AmpliPrep/COBAS ® TaqMan ® HIV-1 Test, version (v) is an in vitro nucleic acid amplification test for the quantitation of Human Immunodeficiency Virus Type 1 (HIV-1) RNA in human plasma using the COBAS ® AmpliPrep Instrument for automated specimen processing and the COBAS ® TaqMan ® Analyzer or COBAS ® TaqMan ® 48 Analyzer for automated amplification and detection. BACKGROUND: HIV type-1 (HIV-1) genetic diversity poses a challenge for the development of diagnostic tests based on nucleic acid amplification, which can be overcome, at least in part, by targeting multiple genomic regions.

The new Roche Diagnostics COBAS TaqMan (CTM) HIV-1 v assay uses multiplex real-time PCR detection of sequences in the long terminal repeat and gag regions within the HIV Cited by: Der in-vitro Real-Time PCR basierte cobas® HIV-1/HIV-2 qual Test eignet sich zur qualitativen Unterscheidung von HIV-1 und HIV-2 in EDTA Plasma oder Serum. Der HIV-1/HIV-2 qual Test beruht auf einer vollautomatisierten Probenvorbereitung (Extraktion und Aufreinigung der Nukleinsäuren), gefolgt von PCR-Amplifikation und Detektion.

The COBAS® TaqMan® HCV Test, v is an in vitro nucleic acid amplification test for the quantitation of Hepatitis C Virus (HCV) RNA genotypes 1 through 6 in human serum or plasma, using the COBAS® AmpliPrep Total Nucleic Acid Isolation Kit (TNAI) for theFile Size: KB.

Wojewoda CM, Spahlinger T, Harmon ML, Schnellinger B, Li Q, et al. () the Roche COBAS TaqMan HIV-1 v assay for determining viral load in Comparison of Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 test version HIV-infected individuals.

Antivir Ther – (CAP/CTM v) with other real-time PCR assays in HIV-1 monitoring and 8. Sloma CR, Germer JJ, Gerads TM, Mandrekar JN. COBAS® AmpliPrep/COBAS® TaqMan® HCV Test 48 Tests P/N: COBAS® AmpliPrep/COBAS® TaqMan® Wash Reagent Liters P/N: INTENDED USE The COBAS AmpliPrep/COBAS TaqMan HCV Test is an in vitro nucleic acid amplification test. Roche said FDA approved its COBAS TaqMan HIV-1 Test v to monitor HIV-1 viral loads in patients. Roche said the test, which has CE Mark approval in Europe, will be available in the U.S.

later. Amplicor HIV-1 Monitor (version ) and the COBAS Ampliprep/Taqman (version ) used in monitoring HIV disease progression in HIV-infected individuals.

Method: In a cross-sectional study, HIV-1 RNA values obtained with the Amplicor HIV-1 monitor version were compared with those of the COBAS/Ampliprep TaqMan HIV-1 version in a routine Author: Olufemi S Amoo, Idowu A Taiwo, Olumuyiwa O Salu, Azuka P Okwuraiwe, Chika K Onwuamah, Morenike A Awe. 1. Kaohsiung J Med Sci. Apr;31(4) doi: /dvfe.uralhimlab.ru Epub Feb 3. Comparison of the Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 test v with v in HIV-1 viral load dvfe.uralhimlab.ru by: 1.

COBAS ® AmpliPrep/COBAS ® TaqMan ® HIV-1 Test, v package insert, Roche Molecular Systems, Document Revision Google Scholar. 3. Mellors JW, Rinaldo Jr CR, Gupta P, White RM, Todd JA, Kingsley LA. Prognosis in HIV-1 infection predicted by the quantity of virus in plasma. Science. ;– PubMed CrossRef Google Scholar. 4. Welles SL, Jackson BJ, Cited by: 4. METHODS: The CAP/CTM HIV-1 Qualitative tests versions and (Roche Molecular Systems, Inc., Branchburg, NJ, USA) were evaluated compared to CAP/CTM TaqMan HIV-1 Quantitative test v (Roche Molecular Systems) on dried plasma spot (DPS) from HIVinfected (with detectable plasma HIV RNA load) and uninfected children, followed-up at Complexe Pédiatrique, Author: CD Mossoro-Kpinde, MA Jenabian, JC Gody, L Robin, P Talla, Jdd Longo, G Grésenguet, L Belec.

A selected group of anti-HIV-1 (+) plasma samples was used for comparison with Roche COBAS Ampliprep/COBAS TaqMan HIV- 1 test v (Roche CAP/CTM), (Roche Molecular Systems, Pleasanton, CA). RESULTS: The 50 and 95 % limit of detection were estimated at (95 % CI ) and () IU/mL respectively. The specificity was found Cited by:   A novel dual HIV-1 target amplification approach was realized in the quantitative COBAS ® AmpliPrep/COBAS ® TaqMan ® HIV-1 Test, v (HIV-1 TaqMan ® test v) to cope with the high genetic diversity of the virus.

Objectives and study design. The performance of the new assay was evaluated for sensitivity, dynamic range, precision, subtype inclusivity, diagnostic and analytical Cited by: Background: HIV-1 viral load assays are critical tools to monitor antiretroviral therapy efficacy in HIV-infected patients. Two assays based on real-time PCR are available, the Abbott Real-Time HIV-1 assay (Abbott assay) and the new Roche COBAS(®) AmpliPrep/COBAS(®) TaqMan(®) HIV-1 test, v. (TaqMan(®) test v).Cited by: When the COBAS TaqMan HIV-1 test is run, the information on the general kit, sample prep.

kit, and PCR kit screens is automatically populated from the TaqMan result file. Below are examples of the Kit Entry module screens after the assay is run showing the data pulled from the TaqMan result file.

To complete the remaining fields, go to. The COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 Test, v is based on three major processes: (1) specimen preparation to isolate HIV-1 RNA; (2) reverse transcription of the target RNA to generate complementary DNA (cDNA), and (3) simultaneous PCR. PQDx WHO PQDx PR May/, version PQDx_ Page 2 of 64 amplification of target cDNA and detection of cleaved. Background High-sensitive real-time PCR assays are routinely used to monitor HIV-1 infected subjects.

Inter-assay discrepancies have been described at the low viral load (VL) end, where clinical decisions regarding possible virological rebound are based. Methods A retrospective study was performed to analyze frequencies of viral blips after transition to the COBAS Ampliprep/COBAS TaqMan v Cited by:   Background.

HIV-1 viral load assays are critical tools to monitor antiretroviral therapy efficacy in HIV-infected patients. Two assays based on real-time PCR are available, the Abbott Real-Time HIV-1 assay (Abbott assay) and the new Roche COBAS ® AmpliPrep/COBAS ® TaqMan ® HIV-1 test, v.

(TaqMan ® test v).Cited by: We evaluated the performance of the cobas® HIV-1 quantitative nucleic acid test for use on the cobas® system ("cobas HIV-1").

METHODS: Limit of detection, linearity, accuracy, precision, and specificity of cobas HIV-1, COBAS® AmpliPrep/COBAS® Taqman® HIV-1 version (CAP/CTM HIV-1 v2) and Abbott RealTime HIV-1 were determined in one or two out of three sites. RESULTS: Cited by: 1. COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 Qualitative Test, v is based on three major processes: (1) specimen preparation to isolate HIV-1 nucleic acids; (2) reverse transcription of target RNA to generate complementary DNA (cDNA), and (3) PCR amplification of target DNA and cDNA, and simultaneous detection of cleaved dual-labeled oligonucleotide detection probes specific to the target.

COBAS AmpliPrep/COBAS TaqMan HIV‐1 v Test (CAP/CTM2) (Roche Molecular System, Inc) for HIV‐1 RNA quantitation. Methods: Assay performance was assessed using clinical samples, a standard HIV‐1 low VL panel, and 2 diluted samples from well‐characterized patients infected with different HIV‐1 subtypes tested in 5 replicates over 3 days. All samples were tested on both assays to Cited by: 1. TaqMan® HIV-1 Test,v and Cobas® TaqMan® HCV Test, v Both of these tests are intended to be used with the High Pure System as a manual extraction method.

Five hundred microliters of each samples and plasma controls from the kit were extracted, using the specific B protocol, on the NucliSENS®easyMag™ (bioMérieux) instrument. Before extraction 7µl of HIV-QS (Quantification. The COBAS TaqMan HIV-1 Test v for use with the High Pure System enables labs to combine an FDA- approved process for manual specimen preparation with an. Abstract Roche modified the COBAS AmpliPrep/COBAS TaqMan human immunodeficiency virus type 1 (HIV‐1) test version (CAP/CTM v), resulting in the COBAS AmpliPrep/COBAS TaqMan HIV‐1 test.

The COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 Qualitative Test, v is an in vitro diagnostic, total nucleic acid amplification test for the qualitative detection of HIV-1 in human EDTA plasma or dried blood spots (DBS) in combination with the COBAS®. The COBAS TaqMan HIV-1 Test, v utilizes the FDA-approved High Pure System Viral Nucleic Acid Kit for manual specimen preparation and the COBAS TaqMan. COBAS TaqMan HIV-1 Test v News Dual-Target-Technologie zur HIVVariantenerkennung Für Labore mit einem mittleren Aufkommen an HIVProben bietet Roche Diagnostics einen.

Mit einer Sensitivität von 20 HIV-1 Kopien/ml erfüllt Cobas Ampli- Prep/Cobas TaqMan HIVTest, v als CE-IVD markierter kommerzieller Test diesen hohen Anspruch. Da der Test einen linearen Bereich von 20 bis Kopien/ml aufweist, kann der HIV-1 Titer auch bis zur Nachweisgrenze quantifiziert werden (Abb. 1). Ermöglicht wird diese Testperformance durch das Dual-Target-Prinzip. In der. A selected group of anti-HIV-1 (+) plasma samples was used for comparison with Roche COBAS Ampliprep/COBAS TaqMan HIV- 1 test v (Roche CAP/CTM), (Roche Molecular Systems, Pleasanton, CA).

Results. The 50 and 95 % limit of detection were estimated at (95 % CI –) and (–) IU/mL respectively. The specificity was found (–) %. Cited by: The COBAS ® AmpliPrep/COBAS ® TaqMan ® HIV-1 Test, v is a nucleic acid amplification test for the quantitation of human immunodeficiency virus type 1 (HIV-1) RNA in human plasma. Specificity Detects and quantitates all known HIV-1 groups M, N, and O, as well as non-B subtypes.

COBAS Ampliprep/COBAS TaqMan HIV-1 Qualitative Test © Frontier Science Foundation 4 May Generate the COBAS TaqMan HIV-1 Qual Test Run Preview Report by clicking on the Report button on the LDMS toolbar.

(See Figure 8.) Figure 8: Run Preview Report Click Select Result File. Click Yes in the Run Assay message that appears. Despite FDA approval and CE marking of commercial tests, manufacturer-independent testing of the technical aspects of newly developed tests is important.

To evaluate the analytical performance and explore the clinical applicability of the new Roche COBAS AmpliPrep COBAS TaqMan HIV-1 test, version (CAP/CTM v), platform comparison was performed with the Roche CAP/CTM test, version Cited by: The COBAS® AmpliPrep/COBAS® TaqMan® HIV-1 Test, version (v) is an in vitro nucleic acid amplification test for the quantitation of Human Immunodeficiency Virus Type 1 (HIV-1) RNA in human EDTA plasma or from a cobas® Plasma Separation Card (PSC) dried plasma spot using the COBAS® AmpliPrep Instrument for automated specimen processing and the COBAS® TaqMan® Analyzer or COBAS.

The COBAS TaqMan HIV-1 Test v for use with the High Pure System enables labs to combine an FDA-approved process for manual specimen preparation with an automated real-time PCR analyzer for amplification and detection. "The availability of this new test will enable more labs, clinicians and patients to have access to Roche's innovative HIV technology," said Whitney Green, senior vice.

Viral load monitoring of HIV-1 has become standard of care in HIV-1 positive patients. In this study, we evaluated the performance characteristics of the Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 test version (CAP/CTM v) in comparison with Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 test version (CAP/CTM v) and Abbott RealTime HIV-1 assay (m), with special Cited by:   Performance evaluation of the new Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 test version for quantification of human immunodeficiency virus type 1 RNA.

Pas S, Rossen JW, Schoener D, Thamke D, Pettersson A, Babiel R, Schutten M. J Clin Microbiol, 48(4), 17 Feb

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